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CellChek® D | D+

Eye Bank Specular Microscopes

Designed to improve patient outcomes, CellChek® D+ redefined specular microscopy in Eyebanking.

It was quickly and widely adopted by many of the world’s finest Eye Banks.

CellChek® D+

Introducing the first multi-imaging system for donor corneal analysis. CellChek® D+ provides an amazing view into the cornea that simply has never been seen before defining the structure of the endothelium, stroma and epithelium. Comprehensive imaging to assist both eye banks and corneal surgeons to make better decisions on implantable material.

Now featured in Cornea June 2018 Supplement: Current and New Technologies in Corneal Donor Tissue Evaluation Comparative Image Atlas. Especially check out S7 “Wide-Field Ex Vivo Dual Imaging Microscopy.”

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Clinical Applications
Donor cornea tissue assessment
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Clinical Benefits

Provides remarkable views of endothelium, intra-stomal structure and the epithelium

For the first time, visualize blood cells, fungus, rough keratome cuts, dead cells, and epithelial anomalies

Regulatory
CE Marked
In Vitro USA

New “Donor Enhanced” Imaging System

CellChek® D+ features a patent applied for “Donor Enhance” imaging system that provides remarkable views of endothelium, intra-stomal structure, and the epithelium. The range of new information from which to make better clinical decisions is spectacular (pun intended): blood cells, fungus, rough keratome cuts, dead cells, epithelial anomalies. Never have we had this view of pre-implanted material. Corneal surgeons are finding this information to be illuminating in helping to understand graft success and failure.

Broad overview but with large multi-sample analytic areas

CellChek® D and D+ feature a new finder feature with digital measuring / documenting tools.

Patented Cellular Analysis Methods

All Konan specular microscopes feature the Center Method of analysis. Center Method is mentioned in FDA panel minutes as being the “gold standard” and is used by virtually every professional reading center for independent assessment of corneal endothelial analytics.¹ Unlike full-auto assessments, the Center Method provides high precision and repeatability for specular images in which relatively small continuous areas of visible cells are visible.

The Flex-Center method is the third tool for advanced stage diseased corneas in which only a very few number of cells are visible. With this semi-automated, perimeter-count method, again, high precision and repeatability is achieved. Only Konan provides the rich set of analytic tools for reliable assessment of the entire spectrum of corneal conditions.

It is not surprising that Konan is the global gold standard in corneal endothelial assessment.

Full Graft Imaging

CellChek® D and D+ provide a total picture of the cornea. Use digital measurement tools to identify, measure and document cornea dimensions, defects, and scars.

Clinical and Scientific Publications

As a corneal specialist for the last 22 years I would feel incomplete without my ability to perform specular in the clinical and eye bank setting. I have Konan units from the earliest to the most recent, all in service currently. They are reliable, accurate, and serve an essential role in my clinical practice and eye bank operations. The service delivered by Konan is some of the best I have experienced in the ophthalmic field. Specular microscopy provides a superb method of clinical screening for disease, an educational training tool for patients, their families and office and eye bank personnel, as well as a tissue evaluative method for eye banking. It is time and cost effective. I wouldn’t want to practice without a Konan specular microscope.

George O. Rosenwasser, MD

Central Pennsylvania Eye Institute - Medical Director, Gift of Life Donor Program Eye Bank

I have used Konan’s eye bank specular microscopes since November 2000. During that time we have processed over 45,000 corneas for transplant. Konan’s microscopes have been proven to be extremely reliable day in and day for a 24/7 operation.

The specular microscope is one of the most vital pieces of equipment for any eye bank. I have enjoyed working with Konan’s professional team as they are always readily available for any question or issue that may arise. I am looking forward to the new CellChek® D and CellChek® D+ which will be the new generation of donor cornea imaging & assessment.

Jason K. Woody

President & CEO, Lions Eye Institute

Enhanced Donor Assessment

Important New Endothelial Detail Discovered with CellChek® D+

Remarkable imaging of non-reflective “grey areas” for more definitive assessment.

CellChek® D+ Provides Visualization of Non-Endothelial Features of the Posterior Cornea

View many features simply invisible to specular only donor imaging:

  • Blood cells
  • Scar tissue
  • Debris
  • Fungus

Image Intra-stomal Keratome and Femtosecond Laser Interface Quality with CellChek® D+

Interface features are invisible to specular-only donor systems.

The Epithelium Now Revealed with CellChek® D+

View many features simply invisible to specular only donor imaging:

  • Blood cells
  • Scar tissue
  • Debris
  • Fungus
What is KonanCare support and clinical training?
KonanCare enables customers to consult, securely, by appointment, with Konan’s on-staff experts for interpretation of specular microscopic images, and to receive assistance on more advanced topics and methods associated with Konan’s products (CellChek® XL, EvokeDx, CellChek® D+ and EB-10, Chart2020, and the RAPDx® pupillograph). With current product models, a secure, permission-based login session can be initiated by the customer allowing both the physician and the KonanCare team member to simultaneously view and discuss actual images using secure internet web services. KonanCare assistance is provided without charge for customers during the initial warranty period or during any period of Service Agreement coverage. A unique service, only from Konan Medical USA.
How accurate are the analysis methods?
Konan's Center & Flex Center methods are patented and are the only FDA 510(k) cleared methods for eye bank systems. Accuracy established by independent reading centers for FDA clinical studies is +/-2.5%
How is the system calibrated?
Each CellChek® D+ comes with a 20um grid calibration disk. Enter 100um length using this disk and click a button. The computer will indicate a known magnification for 100um length. It also enables the operator to verify if calibration was properly completed.
How many cells should be counted to be statistically accurate?
Since it is difficult to determine the degree of cell size and shape variation in donor corneas, most eye banks are marking at least 75-100 cells per cornea. Konan’s multi-sampling capability enables the operator to choose up to 4 different locations in the central cornea for adequate sampling. Analysis from each frame can be automatically averaged.
How do I set a focal distance between the objective lens and the endothelial layer using the different containers, such as vial or chamber?
Since the different containers place the cornea higher or lower than the focal point, by using the proper ring supplied for various containers, the endothelial layer is more or less focused when the top of the black stage and the top of the blue wall are at an even height. There is no need to use any ring for Krolman’s chamber.
What temperature range is required for corneal assessment (after removing from refrigeration)?
CellChek® D+ has a built-in thermometer which measures medium temperature in real-time. For optimal results, the preservation medium should be above 24 – 26 degrees C. (Remember that cornea is the happiest in the body-slightly less than 37 degrees C)
How is the imaging affected with cold corneas and how do we determine if the cornea has warmed up enough to analyze?
At 4º C, biochemical and physiological metabolic activities of endothelial cells slow down significantly. As temperature increases to ~ room temperature (>24º C), the endothelial cell's ability to pump, directly affects the image quality. If cornea has low functional reserve to start with, it may take longer for activation to occur.

Product Specifications


CellChek® D+
EB-10
Viewing Field 1000 x 750 µm 480 X 600 µm
Analysis Area (each multi-field area) 400 X 300 µm X 4 areas (max 480,000 µm2) 200 X 280 µm
Chamber Compatibility Numedis Life 4C®, Krolman®, B+L®, Stephens® Life 4C®, Alcon®, Bausch+Lomb®, Krolman®
Vial Dimensions up to 35 mm diameter up to 35 mm diameter
Thermometer Range 0° to 45° C 0° to 45° C
Stage Translation Ranges X, Y, and Z = 16 mm, Tilt = 15° X and Y = 16 mm, Z= 20 mm, Tilt: 5°
Illumination Halogen LED: primary wavelength 525 nm
Cameras Dual CMOS: Finder and cornea Dual CMOS: Finder and Cornea
Display Digital data feed to computer On-board LCD: temperature and pachymetry
Electrical 100-240 VAC, 50/60 Hz, 50 VA 100-240 VAC, 50/60 Hz, 50 VA
Size 280 (W) X 215 (H) X 265 (D) mm 200 (W) X 255 (H) X 220 (D) mm
Data Interface USB 2.0 USB 2.0
Weight 7.5 kg (without computer) 6.1 kg (without computer)
Operating Conditions Ambient temp: 10° to 40° C
Relative humidity: 30% to 85%
Atmospheric pressure: 70 to 106 kPa		 Ambient temp: 10° to 40° C
Relative humidity: 30% to 85%
Atmospheric pressure: 70 to 106 kPa
Regulatory CE Mark, In Vitro USA CE Mark, In Vitro USA

What Konan Medical Customers Say


The service delivered by Konan is some of the best I have experienced in the ophthalmic field. Specular microscopy provides a superb method of clinical screening for disease, an educational training tool for patients, their families and office and eye bank personnel, as well as a tissue evaluative method for eye banking.

George O.D. Rosenwasser, MD
Central Pennsylvania Eye Institute – Medical Director, Gift of Life Donor Program Eye Bank


I have used Konan’s eye bank specular microscopes since November 2000. Konan’s microscopes have been proven to be extremely reliable day in and day for a 24/7 operation. I have enjoyed working with Konan’s professional team as they are always readily available for any question or issue that may arise. I am looking forward to the new CellChek® D and CellChek® D+ which will be the new generation of donor cornea imaging & assessment.
Jason K. Woody
President & CEO, Lions Eye Institute